solid tissue buffer (Zymo Research)

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Zymo Research solid tissue buffer
Solid Tissue Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+tissue+buffer/Solid+Tissue+Buffer+(Blue)/pm41680255-102-8-11
Average 94 stars, based on 20 article reviews

solid tissue buffer - by Bioz Stars, 2026-09

94/100 stars

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Lysis:

Article Title: Distinct oral-associated gastric microbiota and Helicobacter pylori communities for spatial microbial heterogeneity in gastric cancer
Article Snippet: The frozen tissues were soaked in DNA/RNA Shield Reagent (ZYMO Research) prior to DNA extraction. .. The dissolved tissues were mixed with Solid Tissue Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research), digested with Proteinase K, and disrupted by beads beating (ZR BashingBead Lysis Tube) as previously described ( ). .. The dental plaque samples were mixed with BioFluid and Cell Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research).

Article Title: Use of Metagenomic Reference Materials to Compare Lysis and Extraction Methods Against a Known Input
Article Snippet: .. Metapolyzyme (Sigma-Adrich, Catalog # MAC4L) in combination with a sodium dodecyl sulphate rich buffer, Solid Tissue Buffer (Zymo Research, Catalog # D4086-2-22), proteinase K, and/or bead beating were used for lysis of ZymoBIOMICS Microbial Community Standard (Zymo Research, Catalog # D6300) and stool sample #93 in triplicate. ..

Article Title: Distinct oral-associated gastric microbiota and Helicobacter pylori communities for spatial microbial heterogeneity in gastric cancer.
Article Snippet: The frozen tissues were soaked in DNA/RNA Shield Reagent (ZYMO Research) prior to DNA extraction. .. The dissolved tissues were mixed with Solid Tissue Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research), digested with Proteinase K, and disrupted by beads beating (ZR BashingBead Lysis Tube) as previously described Month XXXX Volume 0 Issue 0 10.1128/msystems.00089-2415 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m sy st em s on 0 5 Ju ly 2 02 4 by 1 03 .1 51 .2 30 .1 59 . (33). .. The dental plaque samples were mixed with BioFluid and Cell Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research).

Incubation:

Article Title: Antibody-mediated immunological memory correlates with long-term Lyme veterinary vaccine protection in mice
Article Snippet: .. All the samples were incubated with Solid Tissue Buffer (Zymo Research Cat. #D4068–2–10) and Proteinase K at 55 °C for 1 h. The DNA was extracted using Quick-DNA 96 kit (Zymo Research Cat. #D3012) following manufacturers’ instructions. .. Quantitative Real-Time PCR was performed using iTaq Universal SYBR Green (BioRad Cat. #1725121), an Applied Biosystem StepOne Plus instrument and primers for Borrelia bb23S and mouse β-actin genes ( ).

Binding Assay:

Article Title: Genome‐Wide Association Study (GWAS) for Linear Body Measurements of Sussex Cattle ( Bos taurus ) at Weaning and Yearling Age in South Africa
Article Snippet: The DNA was obtained using the Zymo Research Quick‐DNA Miniprep Plus Kit (Zymo Research, USA) following the manufacturer's protocol and quantified using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific). .. Briefly, ∼25 hair root samples were placed in a micro‐centrifuge tube and a solution of 95 μL water, 95 μL solid tissue buffer and 10 μL proteinase K was added before mixing and incubating at 55°C for 1–3 h. Two volumes of genomic binding buffer were then added to the supernatant and mixed for 15 s. The mixture was transferred to a Zymo‐Spin llC‐XLR column in a collection tube and centrifuged at ≥ 12,000 × g for 1 min four times. ..

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